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Literature summary extracted from

  • Kamoda, S.; Terada, T.; Saburi, Y.
    Production of heterogeneous dimer lignostilbenedioxygenase II from lsdA and lsdB in Escherichia coli cells (2005), Biosci. Biotechnol. Biochem., 69, 635-637.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.43 expressed in Escherichia coli Sphingomonas paucimobilis

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.43 Sphingomonas paucimobilis
-
-
-
1.13.11.43 Sphingomonas paucimobilis TMY1009
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.43 QAE anion exchange column chromatography, Butyl Toyopearl 650M column chromatography, and DEAE Toyopearl 650C column chromatography Sphingomonas paucimobilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.43 0.0021
-
 at 30°C, in 50 mM Tris-HCl at pH 8.5, using 4,2'-dihydroxy-3,3'-dimethoxy-5'-(2''-carboxyvinyl)stilbene as substrate Sphingomonas paucimobilis
1.13.11.43 0.0024
-
 at 30°C, in 50 mM Tris-HCl at pH 8.5, using 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene as substrate Sphingomonas paucimobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.43 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2
-
 Sphingomonas paucimobilis vanillin
-
 ?
1.13.11.43 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2
-
 Sphingomonas paucimobilis TMY1009 vanillin
-
 ?
1.13.11.43 4,2'-dihydroxy-3,3'-dimethoxy-5'-(2''-carboxyvinyl)stilbene + O2
-
 Sphingomonas paucimobilis ?
-
 ?
1.13.11.43 4,2'-dihydroxy-3,3'-dimethoxy-5'-(2''-carboxyvinyl)stilbene + O2
-
 Sphingomonas paucimobilis TMY1009 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.43 heterodimer
-
 Sphingomonas paucimobilis

Synonyms

EC Number Synonyms Comment Organism
1.13.11.43 lignostilbenedioxygenase II
-
 Sphingomonas paucimobilis
1.13.11.43 LSD
-
 Sphingomonas paucimobilis